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Archive > Volume 17 Issue 3 > 2010,17(3):292-296. DOI:10.3872/j.issn.1007-385X.2010.3.009 Prev Next

Basic Research

Effects of JWA gene on expression and function of Pglycoprotein in tumor cells

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    Abstract:
    Objective:To study the effects of JWA (ADPribosylationlike factor 6 interacting protein 5, ARL6IP5) gene on expression and function of Pglycoprotein in tumor cells. Methods: JWAshRNA and controlshRNA plasmids were transfected into human choriocarcinoma cell line JAR and human breast cancer cell line MCF7,and flagJWA and flagcontrol plasmids were transfected into etoposide (VP16)resistant JAR cells (JAR/VP16) by liposomemediated transfection assay. JWA and Pglycoprotein expressions were examined by Western blotting analysis. Intercellular retention of rhodamine 123 (Rh123) was determined by FCM. Results: After JWA expression was downregulated by JWAshRNA transfection, Pglycoprotein expression was increased in JAR and MCF7 cells, and Rh123 retention was decreased. After JWA was overexpressed by flagJWA transfection, Pglycoprotein expression was decreased in JAR /VP16 cells and Rh123 retention was increased. Conclusion: JWA gene can regulate the expression and transportation of Pglycoprotein in tumor cells.

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    Project Supported:
    Project supported by the National Natural Science Foundation of China (No. 30771829, 30930080)

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