Mobile website
Basic Research
Autocrined VEGF regulates mCRPs expressions in lung cancer cells and the related mechanisms
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore the regulatory effect of autocrined VEGF on membranebound complement regulatory proteins (mCRPs) expression in lung cancer A549 cells and the involved mechanisms. Methods: mRNA expressions of CD46, CD55,CD59, VEGF and their receptors (KDR and FLT1), and IL8 and its receptors (CXCR1 CXCR2) in A549 cells were detected by RTPCR. The effects of antiVEGF antibody and antiIL8 antibody on the proliferation and mCRPs expression in A549 cells were examined by MTT assay and flow cytometry, respectively. The effects of antiVEGF antibody on the expression of transcription factor KLF2 and phosphoNFκB p65 were examined by Western blotting analysis. Results: In addition to membrane CD46, CD55 and CD59 mRNA, both VEGF, IL8 and their receptors (KDR, FLT1; CXCR1, CXCR2) mRNA were expressed in A549 cells. AntiVEGF antibody significantly inhibited the proliferation of A549 cells (P<0.05). CD55 and CD59 mRNA and protein levels in A549 cells were decreased after treatment with 0.1 μg/ml antiVEGF antibody for 72 h (P<0.05). The relative values of KLF2 in cytoplasm and nuclear decreased from 0.63 and 0.88 to 0.42 and 0.66 after treatment with antiVEGF antibody, while those of phosphoNFκB p65 decreased from 0.44 and 0.28 to 0.32 and 019. Conclusion: VEGF may enhance CD55 and CD59 expressions in A549 cells through upregulating expressions of NFκB P65 and KLF2 transcription factors in an autocrine manner.
Keywords:
Project Supported:
Project supported by the Natural Science Foundation of Shandong Province (No. Y2007C154)
Clc Number:
