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Schisandra Chinensis extract prevents radiationinduced lymphocyte reduction in mice and the related mechanisms
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Abstract:
Objective:To study the efficacy of Schisandra Chinensis (SC) extract in prevention of lymphocyte reduction caused by radiation and to explore the related mechanism. Methods: A total of 48 BALB/c mice were divided into 4 groups: SC treated group (SC+ray), NS control group (NS+ray), SC control group (SC), and normal control group (NS). After BALB/c mice were radiated with 6 Gy, the WBC and lymphocyte numbers were counted; T lymphocyte subsets in peripheral blood were detected by immunofluorescence cell staining; morphology of thymocytes was observed by Gimsa staining; lymphocyte subsets and apoptosis rate of thymocytes were examined by flow cytometry; Bcl2 and Fas mRNA expressions in thymus tissues were measured by RTPCR. Results: In NS+ray group, the quantities of WBC, lymphocytes, and lymphocyte subsets were significantly decreased after radiation (P<0.01), but in SC+ray group, the numbers of WBC and lymphocytes were significantly increased compared with those in NS+ray group (P<0.01). The percentages of CD3+, CD4+, and CD8+ T cells in peripheral blood were significantly increased in SC+ray group compared with those in NS+ray group (P<0.01). The quantity of thymocytes was decreased and apoptosis of thymocytes was increased in NS+ray group; cell apoptosis rate in SC+ray group was significantly increased than that in NS+ray group (\[2.87±103\]% vs \[21.32±2.56\]%, P<0.01). The expression of Bcl2 mRNA was significantly downregulated and Fas mRNA was upregulated in NS+ray group (P<0.01); Bcl2 mRNA was upregulated and Fas mRNA was significantly downregulated in SC+ray group compared with those in NS+ray group (P<0.01). Conclusion: SC can prevent radiationinduced lymphocyte reduction through regulating apoptosis of thymocytes, which is mediated by upregulation of Bcl2 and downregulation of Fas.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30772752)
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