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Polypeptide A28 enhances cytotoxic effect of cisplatin on colon cancer cell line HCT116
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Abstract:
Objective:To study the effect of polypeptide A28, which was designed by computer aided drug designing system, on the cytotoxic effect of cisplatin against colon cancer cells. Methods: Colon cancer cell line HCT116 and human umbilical vein endothelial cells (HUVEC) were used in the present study. The concentration of polypeptide A28 was 20 μmol /L and those of cisplatin were 10, 30 and 90 μmol/L. The effects of polypeptide A28 combined with cisplatin on the growth of HCT116 and HUVEC cells were measured by MTT; their effects on the apoptosis of HCT116 cells were examined by flow cytometry. Results：Cisplatin dosedependently inhibited proliferation of HCT116 cells; A28 further enhanced the inhibitory effect of cisplatin on HCT116 cells and increased apoptosis induction effect of cisplatin on HCT116 cells, with the growth inhibition rate of the combination group being (43.3±0.03)%, which was significantly higher than that of the cisplatin single group (15.6±0.10)% (P<0.01). In combination group, when cisplatin concentrations (30, 90 μmol/L) were increased, the inhibitory effects on HCT116 cells were not increased compared with the 10 μmol/L cisplatin combination group (P>0.05). A28 combined with cisplatin (1.1, 3.3, 10, 30, or 90 μmol/L) induced apoptosis of more HCT116 cells than cisplatin single group did (P<0.01). Csplatin at 10 μmol/L combined with A28 at 20 μmol/L effectively killed HCT116 cells, whereas with less toxic effect on HUVEC cells. Conclusion：Polypeptide A28 can enhance the cytotoxic effect of cisplatin on colon cancer cell line HCT116 and decrease its lethal effect on HUVEC.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30772752)
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