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COX2 inhibitor celecoxib induces apoptosis of hepatoma carcinoma SMMC7721 cell line and its mechanism
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Abstract:
Objective:To investigate the effect of COX2 inhibitor celecoxib on induction of apoptosis of hepatoma carcinoma SMMC7721 cell line and the possible mechanism. Methods: SMMC7721 cells were treated with 10, 25, 50, 75 and 100 μmol/L celecoxib; their proliferation was examined by MTT assay; typical apoptotic morphology was studied by Hoechst 33342/PI double staining; and cell cycle and apoptosis rate were detected by flow cytometry. Fas and Bcl2 mRNA expressions were examined by RTPCR, and their protein expressions were detected by Western blotting analysis. Results: Celecoxib dosedependently inhibited the growth of SMMC7721 cells. SMMC7721 cells showed typical apoptotic morphology features, such as nuclear chromatin condensation and nuclear membrane rupture, after celecoxib treatment. Apoptotic rates of SMMC7721 cells treated with 25, 50, and 100 μmol/L celecoxib were (16.32±2.32)%, (38.05±247)%, and (71.17±3.19)%, respectively;celecoxib induced cell cycle arrest of SMMC7721 cells in G0/G1 phase. Fas mRNA and protein expressions in celecoxibtreated SMMC7721 cells were higher than those in the untreated cells (P<001); the expression of Bcl2 mRNA was not affected by celecoxib stimulation (P>0.05), but Bcl2 protein expression was significantly downregulated (P<0.01). Conclusion：Celecoxib can inhibit the proliferation and induce apoptosis of SMMC7721 cells, possibly by regulating the expression of apoptosis related genes.
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Project supported by the Doctor Foundation of Chinese People’s Armed Police Force (No.WB200814)
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