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Antitumor activity of cytokineinduced killer cells against cervical cancer cells in vivo and in vitro
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Abstract:
Objective:To investigate the antitumor activity of cytokineinduced killer cells (CIKs) against cervical cancer cell lines, CasKi and HeLa in vitro and in vivo. Methods: The CIKs were induced from peripheral blood mononuclear cells (PBMCs) of patients with cervical cancer using an improved method, which only used IL2 and antiCD3 antibody, without IFNγ; CIKs were sorted using FACS. The levels of IFNγ, IL2 and TNFα in culture supernatants of CIKs were determined by ELISA. The antitumor activities of CIKs against CasKi and HeLa cells were determined by LDH assay. The nude mouse xenograft models of cervical cancer cell lines, CasKi or HeLa cells, were established, and 1×106 or 1×107 CIKs were administered intravenously once a week for three weeks, then the tumor volumes and weights were measured.Results: CIKs were successfully induced from PBMCs of cervical cancer patients by IL2 and antiCD3 antibody, with CD3+CD56+ cells greatly expanded. CIKs produced significant amounts of IFNγ and TNFα, but few IL2, after PHA stimulation. CIKs dosedependently killed CasKi and Hela cells with a maximum killing rate reaching 43% and 46%, respectively. In addition, the in vivo antitumor experiments demonstrated that CIKs remarkably inhibited the growth of subcutaneous tumors in nude mice (P<0.01). Conclusion: The improved CIKs expansion method used in our study is feasible and the resultant CIKs have remarkable cytotoxicity against Caski and HeLa cells both in vivo and in vitro.
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Project Supported:
Project supported by the Development Foundation for Innovative Talents of Wuhand (No.200997)
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