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Folic acid receptor-mediated targeted transfection of chitosan-pGPU6/GFP/Neo nanoparticles into tumor cells
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Abstract:
Objective:To investigate folic acid receptor-mediated targeted gene delivery of chitosan-pGPU6/GFP/Neo nanoparticles to tumor cells expressing different degrees of folic acid receptors. Methods: Folate-chitosan-pGPU6/GFP/Neo nanoparticles (FA-CS-DNAnano) and chitosan-pGPU6/GFP/Neo nanoparticles (CS-DNAnano) were prepared and identified by infrared spectrometer, and their morphology characteristics and diameters were observed under transmission electron microscope. Human ovarian cancer cell line SKOV3, breast cancer cell line MCF-7, and cervical cancer line HeLa were transfected with the prepared nanoparticles; the transfection efficiency was evaluated by flow cytometry; and cytotoxicities of the nanoparticles were detected by MTT method. Results: FA-CS-DNAnano and CS-DNAnano were successfully prepared. The particles had a smooth surface and a uniform structure, with the diameters being (78.1±0.3) nm and (1384±0.7) nm. Transfection efficiency of FA-CS-DNAnano was significantly higher than that of CS-DNAnano in SKOV3 and MCF-7 cells (\[24.3±0.7\]% vs \[0.7±0.1\]%, \[16.8±1.2\]% vs \[0.3±0.1\]%; all P<0.01), but not in HeLa cells (P>0.05). The cell vitalities of MCF-7, SKOV3 and HeLa cells were (87.9±2.4)%, (91.4±10)%, (97.4±1.1)% before transfection, and were (63.0±2.5)%, (90.6±1.3)%, and (99.3±1.6)% after transfection with FA-CS-DNAnano. Conclusion: Chitosan modified with folic acid is an efficient target gene carrier for folic acid receptor highly positive SKOV3 and MCF-7 tumor cells.
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Project Supported:
Project supported by the Medical Science and Technology Research Foundation of Guangdong Province (No. B2010247); and the Start-up Foundation for Doctorate Candadites of Guangdong Medical College (No. B2009011)
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