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Adenovirus-mediated IL-24 gene induces apoptosis of human glioma U87MG cells
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Abstract:
Objective: To investigate the inhibitory effect of adenovirus-mediated IL-24 (Ad-IL-24) on human glioma U87MG cells and to explore the underlying molecule mechanism. Methods: U87MG cells were infected with Ad-IL-24 constructed in our department, and IL-24 gene expression in U87MG cells was detected by RT-PCR. The growth and apoptosis of U87MG cells were examined by MTT and flow cytometry; the morphological change of U87MG cells was observed by laser scanning confocal microscopy; the Bax and Bcl-2 mRNA expressions were analyzed by RT-PCR; and the activation of caspase-3 was examined by Western blotting analysis. Results: The IL-24 gene was expressed in Ad-IL-24-infected U87MG cells. Ad-IL-24-infection inhibited the growth and induced apoptosis of U87MG cells, which showed typical morphological changes of apoptotic nuclei. Moreover, Ad-IL-24 increased Bax and decreased Bcl-2 mRNA expression, and induced caspase-3 activation in U87MG cells. Conclusion: Ad-IL-24 can induce apoptosis of U87MG cells, probably through up-regulating Bax expression, down-regulating Bcl-2 expression, and inducing caspase-3 activation.
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Project Supported:
Project supported by the Basic Research Foundation of Natural Science in Higher Institutions of Jiangsu Province (No. 08KJB310011)
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