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5-Aza-CdR induced expression of NY-ESO-1 antigen in tumor cells
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Abstract:
Objective:To study the role of a demethylating agent, 5-Aza-2′-deoxycytidine (5-Aza-CdR),in inducing NY-ESO-1 antigen expression in tumor cells. Methods: Human gastric cancer cell line SGC-7901, hepatoma cell lines H2P and MHCC97-H, colon cancer cell lines HT-29 and LoVo, and glioma cell line U251 were used in the present study. NY-ESO-1 mRNA and protein expressions were detected by RT-PCR and immunocytochemistry in the above cell lines before and after 5-Aza-CdR treatment. Results: NY-ESO-1 antigen was positive only in gastric cancer cell line SGC-7901 as detected by RT-PCR and immunocytochemistry. The morphology and growth of hepatoma cell H2P, colon cancer cell LoVo and glioma cell U251 were not obviously affected after 5-Aza-CdR treatment (1 μmol/L, 5 μmol/L and 10 μmol/L ) for 6 days; however, 5-Aza-CdR induced NY-ESO-1 mRNA and protein expressions in these cell lines in a dose-dependent manner. Conclusion: 5-Aza-CdR can induce the expression of NY-ESO-1 antigen in hepatoma, colon cancer and glioma cells, which casts new lights on tumor immunotherapy.
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Project Supported:
Project supported by the Natural Science Foundation of Fujian Province(No.C0610023), and the Foundation for Professors in Fujian Medical University (No.JS08006)
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