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Influence of signal regulatory protein α on adhesion, invasion and apoptosis of breast cancer cell line MDA-MB-231
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Abstract:
Objective : To observe the effects of signal regulatory protein α (SIRPα) on the adhesion, invasion and apoptosis of human breast cancer cells, and to explore the possible mechanism. Methods: SIRPα protein expression in high invasion breast cancer MDA-MB-231 cells and low invasion breast cancer MDA-MB-435 cells were detected by Western blotting analysis. pcDNA3.0-SIRPα plasmid was transfected into MDA-MB-231 cells by lipofectant assay, and SIRPα mRNA expression was examined by RT-PCR. Apoptosis of cells was examined by TUNEL method; invasion and adhesion abilities of MDA-MB-231 cells were examined by invasion or adhesion assays; and JNK and p-JNK protein expressions were determined by Western blotting analysis. Interaction of SIRPα with SHP2 in MDA-MB-435 cells stimulated with EGF was determined by immunoprecipitation assay. Results: Highly invasive MDA-MB-231 cells did not express SIRPα, while lowly invasive MDA-MB-435 cells expressed high level of SIRPα protein. pcDNA3.0-SIRPα transfection enhanced the adhesion of MDA-MB-231 cells, decreased their invasion ability, and promoted their apoptosis. Phosphorylation of JNK in pcDNA3.0-SIRPα transfected MDA-MB-231 cells was also decreased. EGF stimulation further increased SIRPα protein expression in MDA-MB-435 cells and enhanced the interaction of SIRPα with SHP2. Conclusion: SIRPα is related to adhesion and invasion of breast cancer cells, and might promote their apoptosis by decreasing the phosphorylation of JNK.
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Project supported by the National Natural Science Foundation of China （No. 30901805/H3102）
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