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Triterpenes compound extracted from cortex periplocae inhibits tumorigenesis of esophageal cancer Eca109 cells in nude mice and related mechanisms
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Abstract:
Objective : To investigate the inhibitory effect of triterpenes compound extracted from cortex periplocae (TCPP) on tumorigenesis of luciferase-marked Eca109 cells (Eca109, human esophageal cancer cell line; Eca109-luc) in nude mice and the related mechanisms. Methods: Eca109-luc-implanted tumor model was prepared by subcutaneously injecting Eca109-luc cells into nude mice. After treated with TCPP, the volume and weight of transplanted tumors in nude mice were measured, and tumor growth was monitored with in vivo imaging system. Morphology of transplanted tumor tissues was observed by H-E staining; apoptosis of transplanted tumor cells was analyzed by flow cytometry; and survivin expression in transplanted tumor tissues was examined by Western blotting analysis. Results: The growth of implanted tumor in nude mice was markedly inhibited by TCPP, and the volume and weight of tumors were significantly decreased, with the inhibitory rate being 40.7%. TCPP treatment induced inflammatory cell infiltration and necrosis of tumor cells in implanted tumor tissues, and the apoptosis rate of tumor cells was significantly higher than that in soybean oil control group (P<0.05). Expression level of survivin protein in implanted tumors was significantly lower in TCPP-treated group than that in control group (P<0.05). Conclusion: TCPP can inhibit proliferation of human esophageal cancer Eca109 cells in vivo, which might be associated with the down-regulation of survivin and apoptosis of tumor cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China(No. 30772752), and the Natural Science Foundation of Hebei Province (No. C2008000952)
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