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IL-2, IFN-α and IFN-γ up-regulate B7-H1 expression in renal clear cell carcinoma 786-0 cells
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Abstract:
Objective : To study the effects of IL-2, IFN-α and IFN-γ on B7-H1 expression in renal clear cell carcinoma 786-0 cells. Methods: 786-0 cells were stimulated with IL-2, IFN-α and IFN-γ; the expression of B7-H1 mRNA was examined by RT-PCR; and the expression of B7-H1 protein was detected by immunocytochemistry staining, immunofluorescence staining, and flow cytometry assay. Results: RT-PCR results showed that B7-H1 mRNA expression was higher in 786-0 cells stimulated with IL-2, IFN-α, and IFN-γ compared with those in unstimulated cells (0.75±0.06,0.68±005, 0.95±0.08 vs 0.30±0.03, P﹤0.05). B7-H1 protein was distributed in the cell membrane as showed by immunocytochemistry and immunofluorescence staining, and the expression of B7-H1 protein was up-regulated after IL-2, IFN-α, and IFN-γ stimulation. Flow cytometry results showed that B7-H1 protein expressions in 786-0 cells stimulated with IL-2, IFN-α, and IFN-γ were significantly higher than that in unstimulated cells (\[65.70±3.26\]%, \[56.52±175\]%, \[84.05±3.52\]% vs \[20.49±1.03\]%, P<0.01). Conclusion: IL-2, IFN-α and IFN-γ can all up-regulate the expression of B7-H1 mRNA and protein in 786-0 cells, with IFN-γ showing the most potent up-regulation effect and IFN-α showing the least one.
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