Mobile website
Basic Research
Isolation and purification of polysaccharide from Se-enriched spirulina platensis and their in vitro anti-tumor activity
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective : To investigate the effects of different polysaccharides isolated from Se-enriched spirulina platensis (Se-PSP) on the growth of tumor cells. Methods: Crude total sugar Se-PSP and its different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) were extracted from se-enriched spirulina (Se-SP) by ultrasonic method, and four Se-PSP components were further isolated and purified by DEAE-cellulose column. The effects of total sugar Se-PSP and four different components (10 μg/ml) on the growth of SKOV-3 cells, and different concentrations of Se-PSP2 on the growth of SKOV-3 cells, HepG-2 cells, BGC-803 cells, CNE cells and Tca-8113 cells were examined by MTT method. Results: Four different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) were isolated and purified from Se-PSP, with Se-PSP2 being the most abundant one. The inhibitory rates of Se-PSP and four different components (Se-PSP1, Se-PSP2, Se-PSP3 and Se-PSP4) on growth of SKOV-3 cells were (33.393±5.437)%, (22.290±8.399)%, (44.881±4.878)%, (45.354±4931)% and (46.203±4.455) %, respectively, with the inhibitory rates of Se-PSP2, Se-PSP3 and Se-PSP4 being significantly higher than those of Se-PSP and Se-PSP1 (P<0.05), but those of Se-PSP2, Se-PSP3 and Se-PSP4 showing no difference (P>0.05). Se-PSP2 dose-dependently inhibited the proliferation of SKOV-3, HepG-2, BGC-803 and Tca-8113 cells (all P<0.05), and showed broad anti-tumor spectra. Conclusion: Se-PSP2 is the main component of Se-PSP, and it can inhibit the growth of various types of tumor cells, making it a promising adjuvant drug for treatment of malignant tumors.
Keywords:
Project Supported:
Project supported by the Innovation Education Foundation for Postgraduates (No. 2007105981002M07)
Clc Number:
