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promotes apoptosis of human pancreatic carcinoma Aspc1 cells
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Abstract:
Objective : To investigate the effect IL-27 on apoptosis of human pancreatic carcinoma Aspc1 cells and its in vivo anti-tumor activity. Methods: PA317/IL-27 retrovirus vector was transfected into Aspc1 cells and the stable clones (Aspc1/IL-27) were obtained by selecting with G418. The effects of IL-27 on production of IL-27, proliferation, and MHC-Ⅰ expression of Aspc1 cells were determined by ELISA, cell counting and flow cytometry, respectively. Aspc1/IL-27, Aspc1/LXSN (Aspc1 cells stably transfected with empty vector) and Aspc1 cells were subcutaneously injected into nude mice, and the growth of transplanted tumors and survival time of mice were observed. Apoptosis and ultramicrostructure of the implanted-tumor cells were examined by TUNEL and electron microscope respectively. Results: Aspc1 cells stably transfected with PA317/IL-27 (Aspc1/IL-27) were successfully prepared. Aspc1/IL-27 cells secreted high levels of IL-27, while Aspc1/IL-27 and Aspc1 cells did not secreted IL-27 (P<0.01). Aspc1/IL-27 transfection did not affect the expression of MHC-Ⅰ on Aspc1 cells (P>0.05). The growth of implanted-tumors was significantly slower and the survival time was longer in Aspc1/IL-27 group than those in Aspc1/LXSN and Aspc1 groups (P<0.05). Apoptosis rate of implanted-tumor cells in Aspc1/IL-27 cells was significantly higher than those in Aspc1/LXSN and Aspc1 groups (\[19.5±2.4\]%, \[8.5±0.3\]%, \[9.1±0.8\]%, P<0.01) . Conclusion: IL-27 gene transfection exerts in vivo anti-tumor activity by inducing apoptosis of human pancreatic carcinoma cells.
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Project Supported:
Project supported by the Scientific Research Foundation of Science and Technology Bureau of Hebei Province (No.10276105D-98), and the Scientific Research Foundation of Health Bureau of Hebei Province (No. 20100413)
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