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Heparanase promotes invasion and adhesion of ovarian carcinoma cells
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Abstract:
Objective : To explore the roles of heparanase (HPSE) in the invasion and metastasis of human ovarian carcinoma A2780 cells. Methods: pcDNA3.1-HPSE eukaryotic expression vector was constructed. pcDNA3.1-HPSE and empty pcDNA3.1 plasmids were transfected into A2780 cells by Lipofectamine method, and A2780 cells stably expressing pcDNA3.1-HPSE or pcDNA3.1 were selected by G418 (named pcDNA3.1-HPSE-A2780 or pcDNA3.1-A2780 cells). The proliferation of A2780 cells after pcDNA3.1-HPSE transfection was detected by MTT and clone-forming experiment, and the invasion, migration and adhesion capacities of A2780 cells were tested by Matrigel, Transwell and Adhersion assays, respectively. Results: The pcDNA3.1-HPSE vector was successfully constructed and transfected into A2780 cells. pcDNA3.1-HPSE transfection had no effect on proliferation and migration of A2780 cells (all P>0.05). pcDNA3.1-HPSE transfection increased invasion (0.477±0.024 vs 0.250±0.081, P=0.003) and inhibited the adhesion of A2780 cells (0.728±0.089 vs 0.518±0.080, P=0.002). Conclusion: Heparanase plays important roles in ovarian carcinoma by promoting invasion and inhibiting adhesion of tumor cells.
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Project Supported:
Project Supported by the Guangxi Natural Science Foundation of Science and Technology Bureau in Guangxizhuang Autonomous Region (No. 2010GXNSFA013142)
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