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MicroRNA-17-5p specific antisense oligonucleotide inhibits cell cycle of leukemia K562 cells
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Abstract:
Objective : To study the effect of microRNA-17-5p (miR-17-5p) on the growth of chronic myelocytic leukemia K562 cells by antisense oligonucleocide technique. Methods： miR-17-5p antisense oligonucleotide (miR-17-5p-ASO) and control nonsense oligonucleotide (Ctrl-NSO) were transfected into K562 cells by Lipofectamine assay, and un-transfected K562 cells were used as blank group (Ctrl). The proliferation, apoptosis, and cell cycle changes of K562 cells were examined by MTT, TUNEL, and flow cytometry assays, respectively. Results: MTT results showed that the proliferation of K562 cells in miR-17-5p-ASO group was (61.7±4.7)% of that in Ctrl-NSO group, and miR-17-5p-ASO transfection significantly inhibited the proliferation of K562 cells (P<0.05). TUNEL results showed that miR-17-5p-ASO transfection did not affect apoptosis of K562 cells (P>0.05). The ratio of K562 cells in G2 phase was (10.8±08)％ in miR-17-5p-ASO group, which was significantly lower that those in Ctrl-NSO and Ctrl groups (\[34.6±04\]%, \[33.9±1.3\]%, all P<0.05). Conclusion: MiR-17-5p specific antisense oligonucleotide can suppress K562 cells growth by inhibiting cell cycle, which may provide a new way for treatment of leukemia.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.30873017)
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