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Astragalus polysaccharide promotes maturation of plasmacytoid dendritic cells of patients with acute myeloid leukemia
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Abstract:
Objective : To explore the effect of astragalus polysaccharide (APS) on maturation of plasmacytoid dendritic cells (pDCs) from acute myeloid leukemia (AML) patients. Methods: Twenty-three AML patients from Second People’s Hospital of Huai’an City (Oct. 2009 to Feb. 2010) were used in this study. Peripheral mononuclear cells were obtained and were sorted by flow cytometry. pDCs were then stimulated with APS for 5 days; their phenotypes were analyzed by flow cytometry, morphology was observed under light microscope, and ultrastructure was observed under scanning electron microscope. pDCs were treated with different concentrations of APS (0, 50, 100, 200 μg/ml) for 24 h, and IFN-α, TNF-α and IL-6 levels in pDC supernatants were examined by ELISA. Results: Light microscope and scanning electron microscope results showed that APS-treated pDCs displayed mature morphology with more and longer dendritic spines. APS promoted differentiation and maturation of pDC by increasing the positive rates of CD11c, CD80 and CD86 in a dose-dependent manner (P＜0.05). APS also dose-dependently up-regulated IFN-α, TNF-α, and IL-6 secretion in pDCs (P＜005). Conclusion: APS can enhance the function of pDCs of AML patients, and promote their differentiation and maturation.
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