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Xiaoai Jiedu Recipe inhibits transplanted hepatocarcinoma H22 tumors and the related mechanism
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Abstract:
Objective:To study the inhibitory effects of Xiaoai Jiedu Recipe(XJR) against transplanted hepatocarcinoma H22 tumors in mice and the related mechanism. Methods: H22-transplanted tumor mouse models were established and were divided into control group, XJR treatment groups (10, 30, 90 g/kg), and cisplatin treatment group. The growth of tumors in different groups was measured, and pathology changes of transplanted tumor tissues in different groups were examined by H-E staining. Flow cytometry was applied to examine cell cycle and sub-diploid apoptosis rate of transplanted tumor cells, and VEGF levels in the peripheral blood of mice were measured by ELISA. Results: Compared with control group, different XJR groups and the cisplatin group significantly inhibited growth of H22-transplanted tumors (245%, 42.8%, 21.1, 58.6% vs 0, P<0.05, P<0.01), cells in XJR groups showing massive necrosis and obvious chromosome condensation. Medium-dosage XJR and cisplatin blocked transplanted tumor cells in G0/G1 phase (P<005), and the cells in S phase were significantly reduced (P<0.05). The sub-diploid apoptosis rate of transplanted tumor cells in medium-doseage XJR group was similar to that in cisplatin group (60.52±6.40 vs 71.32±16.02, P>0.05). Peripheral VEGF levels were significantly lower in the medium-, large-dosage XJR groups and cisplatin group than in the control group (104.3±6.1, 105.8±7.2, 88.6±4.3 vs 120.7±12.6, P<0.05, P<0.01). Conclusion: Xiaoai Jiedu Recipe can inhibit the growth and promote apoptosis of H22-transplanted tumor cells, and inhibition of VEGF may be one of the mechanisms for the anticancer effect.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30973742/C190201), the Natural Science Foundation of Jiangsu Province (No. BK2007240), the Natural Science Foundation of Higher Institutions in Jiangsu Province (No. 09KJB360003), the Science Inovation Foundation for Graduates in Common Higher Institutions of Jiangsu Province (No. CX09S_037Z), and the Science Foundation from the Administration of Traditional Chinese Medicine in Jiangsu Province (No. LB09032)
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