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Snail increases resistance of breast cancer MCF-7 cell transplanted tumors to doxorubicin and its mechanism
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Abstract:
Objective:To study the effect of Snail on resistance of breast cancer MCF-7 cell transplanted tumors to doxorubicin and its possible mechanism. Methods: Snail eukaryotic expression vector pcDNA3.1-Snail was constructed and transfected into MCF-7 cells, and MCF-7 cells with stable Snail expression (MCF-7/Snail cells) were screened. MCF-7 cells transfected with blank pcDNA3.1 (MCF-7/pcDNA cells) were used as control. MCF-7/Snail- and MCF-7/pcDNA-cell transplanted tumor models were established. After doxorubicin injection, the growth of transplanted tumors was observed, and the inhibitory rate of doxorubicin was calculated. The expressions of Snail, MDR-1 and MMP-9 in transplanted tumor tissues were examined by immunohistochemistry. Results: pcDNA3.1-Snail expression vector was successfully constructed, and MCF-7/Snail and MCF-7/pcDNA cells were obtained. After doxorubicin therapy, the transplanted tumor weight in MCF-7/Snail group was significantly higher than that in the MCF-7/pcDNA group (\[1.413±0674\]g vs \[1.257±0576\]g, P<0.05), and the inhibitory rate of doxorubicin was significantly lower (18.42% vs 30.18%, P<0.05). The expressions of Snail, MDR-1 and MMP-9 in transplanted tumor tissues were significantly higher than those in MCF-7/pcDNA group (408.08±20.39 vs 67.67±16.56, 363.50±26.56 vs 55.08±12.23, 396.25±16.03 vs 56.92±7.35, all P<0.05)，and the expression of Snail was positively correlated with that of MDR-1 and MMP-9 (r1=0.89, P<0.01; r2=0.81, P<0.01). Conclusion:Snail can increase resistance of breast cancer MCF-7 cell transplanted tumors to doxorubicin, which might be related with the increased expressions of MDR-1 and MMP-9 in breast cancer MCF-7 transplanted tumors.
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Project Supported:
Project supported by the Science and Technology Program of Higher Institutions of Shandong Province (No. J10LF64)
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