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Detection of Th17 cells in peripheral blood of esophageal cancer patients and its clinical significance
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Abstract:
Objective:To study the ratio of Th17 cells in peripheral lymphocytes and the expression of RORγt and IL-17 in the peripheral blood mononuclear cells (PBMCs) of patients with esophageal squamous cell carcinoma (ESCC), and to investigate their clinical significance. Methods: Forty PBMC samples from ESCC patients (24 men and 16 women, mean age 61±8.76 years), who were diagnosed in Qilu Hospital of Shandong University from August 2009 to June 2010, and 40 healthy volunteers were included in the present study. The ratio of Th17 cells in peripheral lymphocytes was detected by flow cytometry, and the expression levels of IL-17 and RORγt mRNA in PBMC were examined by PT-PCR. Results: The ratio of Th17 cells in the peripheral lymphocytes of ESCC patients was significantly higher than that in healthy volunteers (\[2.40±0.55\] % vs \[0.84±0.41\]%, P<0. 01). Both RORγt and IL-17 mRNA expression levels in ESCC patients were significantly higher than those in the healthy volunteers (\[0.669±0.184\] vs \[0.451士0.151\], \[0.625±0.179\] vs \[0.438±0.150\], P<0.01), and the two showed a positive correlation (r2 =0.551, P<0.01). In addition, the Th17 ratio, and RORγt and IL-17 mRNA expression levels were significantly higher in metastasis ESCC patients compared with those in the non-metastasis ESCC patients (P<0.05). Conclusion: Th17 ratio and RORγt and IL-17 mRNA expression levels are significantly increased in ESCC patients, and Th17 cells may participate in the development of ESCC through RORγt and IL-17.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30571844), the Science and Technology Development Foundation of Shandong Province (No. 2009GG10002007), and the Natural Science Foundation of Shandong Province (No. ZR2009CM090)
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