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Clinical Research
Human glioma expresses high level of Snail and promotes tumor cell invasiveness
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Abstract:
Objective:To investigate the expression of Snail protein in human glioma tissues and study the effect of Snail on human glioma U251 cells. Methods: Sixty-five specimens from glioma patients, who were diagnosed in Beijing Anzhen Hospital and Beijing Tiantan Hospital, were included in this study. Immunohistochemistry S-P was used to detect Snail protein expression in human glioma tissues. Snail specific small interference RNA (Snail-siRNA) was constructed and transfected into U251 cells by lipofectamine. The expressions of Snail mRNA and protein and E-cadherin protein in transfected-U251 cells were investigated by RT-PCR and Western blotting analysis, respectively; and the invasion ability of transfected-U251 cells was investigated by Transwell chamber assay. Results: The positive rate of Snail was 662% in human glioma, which was significantly higher than that in the normal brain tissues (0%, P<0.01), and Snail positive rate in Ⅰ-Ⅱ stage glioma was significantly lower than that in Ⅲ-Ⅳ stage glioma (44.8% vs 83.3%, P<001). Snail-siRNA transfection inhibited the expressions of Snail mRNA and protein. The expression of E-cadherin protein in Snail-siRNA transfected-U251 cells was significantly increased compared with those in Ctrl-siRNA and untransfected groups (064±0.21 vs 0.15±0.16, 0.21±0.19, P<0.01). Snail-siRNA transfection inhibited the invasion of U251 cells （87.0±2.4 vs 140.0±4.9, 136.0±5.3; P<0.05）.Conclusion: Snail protein is highly expressed in human glioma tissues, and siRNA interfering the expression of Snail protein can inhibit invasion of glioma U251 cells.
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