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Inhibitory effect of Newcastle disease virus 7793 strain on human colon carcinoma LoVo cell-transplanted tumors in nude mice and the possible mechanism
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Abstract:
Objective:To investigate the effects of Newcastle disease virus 7793 strain (NDV 7793) on the growth of human colon carcinoma LoVo cell-transplanted tumors in nude mice and the possible mechanism. Methods: Mouse models of LoVo cell-transplanted tumor were established and were randomly divided into 3 groups: intravenously injected with PBS, 5-FU and NDV 7793 groups. Tumor growth was observed in different groups, the apoptosis and necrosis rates of tumor cells were detected by FCM, expressions of Bax and Bcl-2 proteins were analyzed by immunohistochemical method, cyto-C level in tumor tissues was detected by cyto-C kit, and the concentration of TNF-α in tumor tissues was examined by ELISA. Results: NDV 7793 significantly inhibited the growth of LoVo-transplanted tumors compared with 5-FU (50.14% vs 3714%, P<0.05). The apoptosis rate of LoVo-transplanted tumor cells in NDV 7793 group was significantly higher than that in 5-FU group (\[28.7±1.5\]% vs \[1.46±0.3\]%), and LoVo-transplanted tumor cells had a similar apoptosis rate and necrosis rate in NDV 7793 group (\[28.7±1.5\]% vs \[27.80±3.32\]%). NDV 7793 enhanced the expression of Bax, but not Bcl-2, in LoVo-transplanted tumor tissues, NDV 7793 also increased the cyto-C (\[2.28±0.68\] vs \[0.68±0.13\] μg/μl) and TNF-α levels (\[489.6±5.2\] vs \[167.9±3.9\] pg/ml) in LoVo-transplanted tumor tissues. Conclusion：NDV 7793 can inhibit the growth of human colon carcinoma LoVo cell-transplanted tumors, which may be related to the up-regulation of Bax, cyto-C and TNF-α and the subsequent apoptosis of tumor cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30860328), and the Scientific Research Program of Education Bureau of Guangxi Province (No. 200810MS059)
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