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Honokiol induces apoptosis of human acute myeloid leukemia KG1a cells
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Abstract:
Objective:To investigate the apoptosis-inducing effect of honokiol (HNK) on human acute myeloid leukemia KG1a cells and the possible mechanism. Methods: KG1a cells were treated with different concentrations of HNK, and then the proliferation of KG1a cells was detected by XTT assay. Flow cytometry was performed to examine the cell cycle and apoptosis of KG1a cells after HNK (0, 5, 10 μg/ml) treatment. RT-PCR technique was used to detect the expression of apoptosis-related genes (〖STBX〗Bcl-2, Bid, Bax, Bak, Bad, P53 and NF-κB〖STBZ〗) in KG1a cells. Results: HNK (2.5, 5, 8, 10, 15, 20, and 40 μg/ml) significantly inhibited the growth of KG1a cells in a time- and dose-dependent manner (P<0.01), and IC50 concentrations in 24 h, 48 h were 10.23 μg/ml and 8.25 μg/ml, respectively. Flow cytometry results revealed that KG1a cells were arrested at G0/G1 phase after treated with HNK; the early apoptotic rates of KG1a cells after HNK treatments (5 μg/ml and 10 μg/ml) were significantly higher than those in control group (0 μg/ml), with apoptotic rates being (\[11.16±1.27\]%, \[21.46±3.13\]% vs \[6.03±1.10\]%, P<0.01). Meanwhile, RT-PCR revealed the mRNA expression of apoptosis-promoting gene Bax was significantly increased, with Bad slightly improved after HNK treatments compared with control group; meantime, the apoptosis-inhibiting gene (NF-κB) was markedly decreased. Conclusion: HNK can induce apoptosis of KG1a cells, which might be related to increased expression of Bax, Bad genes, and decreased expression of NF-κB gene.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.30973454)
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