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Cisplatin-resistance increases proliferation, invasion and migration of ovarian cancer OVCAR-3 cells
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Abstract:
Objective:To investigate the differences in biological behavior between cisplatin-sensitive (OVCAR-3 cells) and cisplatin-resistant human ovarian cancer cells (OVCAR-3/CDDP cells), and to explore the possible mechanisms. Methods: OVCAR-3 and OVCAR-3/CDDP cells in logarithmic phase were collected, their proliferation were assessed by soft agar colony formation assay, their in vitro and in vivo invasion and migration abilities were measured by Transwell chamber assay, anoikis assay and subcutaneous transplanted-tumor formation assay in nude mice, and the expressions of MMP-2 and MMP-9 in transplanted tumor tissues were examined by immunohistochemical assay. Results: Colony formation rate of OVCAR-3/CDDP cells was significantly increased compared with OVCAR-3 cells (\[0.66%±009%\] vs \[0.31%±0.07%\], P<0.05). Compared with OVCAR-3 cells, OVCAR-3/CDDP cells had significantly higher migration capability (\[233.1±8.5\] vs \[167.4±5.9\], P<0.01) and invasive capability (\[143.6±9.1\] vs \[95.8±6.2\], P<0.01). OVCAR-3/CDDP cells tended to aggregate and their apoptosis index was decreased compared with that OVCAR-3 cells (\[7.78±1.32\]% vs \[15.41±1.26\]%, P<0.01). The expressions of MMP-2 and MMP-9 in transplanted tumor tissues of OVCAR-3/CDDP cell group were up-regulated compared with those of OVCAR-3 cell group (P<0.05). Conclusion: The proliferation, anti-anoikis, invasion and migration abilities of cisplatin-resistant OVCAR-3/CDDP cells are greatly increased, which might be related with the up-regulation of MMP-2 and MMP-9.
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Project Supported:
Project supported by Medical Science Research Program of the“11th Five-Year”Plan of PLA (No. MB2151)
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