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Synergistic inhibitory effects of bFGF monoclonal antibody combined with radiotherapy on B16-transplanted tumors in mice
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Abstract:
Objective:To investigate the inhibitory effect of basic fibroblast growth factor monoclonal antibody (bFGF-mAb) combined with radiotherapy against B16-transplanted tumors in mice. Methods: bFGF-mAb was prepared and purified. B16-transplanted melanoma tumor models were established and the mice were randomly divided into 4 groups: control group, radiotherapy group, bFGF-mAb group, and bFGF-mAb combined with radiotherapy group. Tumor volumes were measured in different treatment groups. Twenty days after treatment, the tumors were collected and weighted, and the inhibitory rates of tumor growth were calculated. TUNEL staining was used to detect the apoptosis rate of transplanted tumors; immunohistochemical method was used to examine the positive expression of bFGF, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in transplanted tumor tissues. Results: The inhibitory rate of tumor growth in the combined treatment group was significantly higher than those in radiotherapy group and bFGF-mAb treatment group (48.76% vs 12.17%, 30.49%, P<0.05). The radiotherapy sensitization enhancement ratio of the combined treatment group was 2.37 times that in the radiotherapy group. The apoptosis rate of transplanted tumor cells in the combined treatment group was significantly increased compared with those in the control, radiotherapy, bFGF-mAb treatment groups (\[58.56±6.47\]% vs \[17.21±2.86\]%, \[28.45±5.47\]%, \[10.62±1.73\]%; P<0.05 or P<001), with bFGF, VEGF expression and MVD being significantly decreased (P<005). Conclusion: bFGF-mAb combined with radiotherapy have synergistic inhibitory effect on the growth of B16-transplanted melanoma tumors, and it can increase the radio-sensitivity of tumor cells by reducing the expressions of bFGF and VEGF, decreasing angiogenesis, and promoting apoptosis.
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Project Supported:
Project supported by the National High Technology Research and Development Program (863 Program) of China (No. 2009AA022112)
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