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Inhibitory effect of Fomitopsis pinicola extract on hepatocellular carcinoma H22 cells and sarcoma S180 cells
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Abstract:
Objective:To study the in vitro and in vivo anti-tumor activities of Fomitopsis pinicola extract and their possible mechanisms. Methods: Fomitopsis pinicola was extracted by alcohol and the resulting acid ethyl esters extract was named FP-I. The inhibitory effects of FP-I on proliferation of mouse hepatocellular carcinoma H22 cells and mouse sarcoma S180 cells were detected by MTT assay in vitro. S180-implanted mouse model was established, and the tumor weight, tumor inhibitory rate, thymus index, spleen index, peripheral leukocyte number, peripheral lymphocyte rate, and associate tumor erythocyte rosette rate (ATER) were examined in different group. Effect of FP-I on apoptosis of S180-implanted tumor cells and the histological changes of the heart, liver, kidney, thymus and spleen were observed by H-E staining. Results: The inhibitory rates of FP-I (50, 100, 200, 400 μg/ml) on proliferation of S180 cells were 22.35%, 32.49%, 40.01%, and 74.01%, respectively; and those on H22 cells were 45.19%, 51.10%, 66.37%, and 82.40%, rspectively. The inhibitory rates of FP-I (25, 50, 100 μg/ml) on growth of S180-implanted tumors were 79.92%, 66.18% and 78.45%, respectively, and that of CTX (30 mg/kg) positive control was 84.10%. Proportion of lymphocyte in periphoral blood, thymus index and ATER of S180-bearing mice were significantly increased in both the high-and medium-dose FP-I groups (P<0.05 or P<0.01). S180-implanted tumor cells in all FP-I treatment groups showed typical apoptotic morphology. Conclusion: Fomitopsis pinicola extract has anti-tumor activity, which might relate to its ability to improve the immune function of mice and to induce apoptosis of tumor cells.
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Project Supported:
Project supported by the Research Foundation of Education Bureau of Hunan Province (No. 08C142, No.2009-320-372)
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