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Correlation of Toll-like receptor 4/9 expression with pathological stages and metastases of gastric cancer
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Abstract:
Objective:To investigate the correlation of Toll-like receptors（TLR）4 and TLR9 expression with the pathological stages and metastases of gastric cancer. Methods: Forty-two gastric cancer and paracancerous tissues were included in the present study (patients were diagnosed in No.174 Hospital of PLA from Jan.1 2009 to Oct. 2009). The mRNA and protein expressions of 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 in the gastric cancer and paracancerous tissues were detected by RT-PCR and Western blotting analysis, respectively. Results: The expressions of 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 mRNA were significantly higher in the gastric cancer tissues than those in the paracancerous tissues (1.29±0.03 vs 0.53±0.02, 0.99±0.04 vs 0.22±0.05；P<0.05). T3+T4 gastric cancer tissues demonstrated significantly higher levels of 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 mRNA than the T1+T2 tissues (P<0.05). Significantly higher levels of 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 mRNA were observed in patients with distant metastases than in those without (P<0.05). However, no correlation was observed between 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 mRNA expression with the age, gender or lymphatic metastasis status of patients. The expressions of 〖STBX〗TLR4〖STBZ〗 and 〖STBX〗TLR9〖STBZ〗 protein were significantly higher in the gastric cancer tissues than those in the paracancerous tissues (1.36±0.05 vs 0.48±0.04, 112±0.05 vs 0.34±0.03;P<0.05), T3+T4 gastric cancer tissues demonstrated significantly higher levels of TLR4 and TLR9 proteins than the T1+T2 tissues (P<0.05). Conclusions: TLR4 and TLR9 are up-regulated in gastric carcinoma tissues, and TLR4/9 expression is correlated with the TNM staging and distant metastasis. These data suggest that TLR4/9 may be involved in the development and progression of gastric cancer, and TLR4/9 may be potential target for gastric cancer treatment．
Keywords:
Project Supported:
Project supported by the Scientific and Technology Foundation of Xiamen (No. KJ2010014)
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