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Expression of S100 calcium binding protein A13 in hepatocellular carcinoma and its clinical significance
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Abstract:
Objective:To explore the expression of S100 calcium binding protein A13 (S100A13) in hepatocellular carcinoma (HCC) tissues and its clinical significance. Methods: Thirty-four HCC tissues, 18 corresponding paracancerous tissues, and 9 normal liver tissues were obtained from First Affiliated Hospital of Guangxi Medical University, and the high density chips of 197 HCC tissues and corresponding paracancerous tissues were obtained from commercial company. In situ RT-PCR was used to detect the expression of S100A13 mRNA in HCC tissues, paracancerous tissues, and normal liver tissues. Immunohistochemistry was used to detect the expression of S100A13 protein in HCC tissues, paracancerous tissues, and normal liver tissues. We analyzed the optical density of immunohistochemistry results by Image-Pro Plus. Results: The expression rates of S100A13 mRNA in HCC tissues, their corresponding paracancerous tissues and normal liver tissues were 70.21%, 51.06%, and 33.33%, respectively. S100A13 mRNA was mainly expressed in the nucleus, especially in nuclear membranes. The expression rates of S100A13 protein in HCC tissues, their corresponding paracancerous tissues and normal liver tissues were 55.84%, 38.96%, and 26.32%, respectively. The highest average optical density was found in HCC tissue (0.038±0.051), followed by paracancerous tissues (0.022±0.034), and the lowest was found in normal liver tissues (0.01±0.009) (P＜0.05). S100A13 protein was mainly expressed in the cytoplasm of HCC cells, occasionally in the nucleus; the secondary bile canaliculi and some inflammatory cells also showed high S100A13 protein expression. There was no correlation between S100A13 expression and the sex, age, and tumor grade of HCC patients. Conclusion: S100A13 protein is highly expressed in HCC, and it might serve as a potential target in HCC therapy.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.30760233), and the Natural Science Foundation of Guangxizhuang Autonomous Region of China (No.0728121)
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