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Expressions of 14-3-3σ and cyclin B1 proteins in breast carcinoma and their clinical significances
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Abstract:
Objective:To study the expressions of 14-3-3σ and cyclin B1 proteins in breast carcinoma tissues and to analyze their relationships with clinical characteristics of breast carcinoma. Methods: Ninety-five breast carcinoma tissues and thirty corresponding adjacent tissues (patients were diagnosed in First Affiliated Hospital of Liaoning Medical University from Dec. 2006 to Dec. 2009; 31〖KG-*2〗〖DK〗-85 years old, median age 58 years) were included in the present study. The expressions of 14-3-3σ and cyclin B1 proteins in the breast carcinoma tissues and adjacent tissues were detected by immunohistochemistry PV and Western blotting methods, and their correlation with clinical characteristics of breast carcinoma was analyzed. Results: Immunohistochemistry and Western blotting results showed that the expression rate of 14-3-3σ in breast carcinoma was 22.1% (21/95), which was significantly lower than that in the adjacent tissues (93.3%, 28/30; P<005); the expression of 14-3-3σ was correlated with the histological grade, TNM stage, and lymph node metastasis of breast carcinoma (P<0.05). The expression rates of cyclin B1 in breast carcinoma was 69.5% (66/95), being significantly higher than that in the adjacent tissues (40%, 12/30; P<0.05). The expression of cyclin B1 was only correlated with lymph node metastasis of breast carcinoma (P<005); expression of 14-3-3σ was negatively correlated with cyclin B1 in the breast carcinoma tissues (r=-0.333, P=0.001). Conclusion: 14-3-3σ and cyclin B1 proteins are related to the progression and metastasis of breast carcinoma, and their combined detection may help the diagnosis and treatment of breast carcinoma.
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Project Supported:
Project supported by Science and Technology Foundation of Liaoning Province(No.2010225034), and the Scientific Foundation of Education Bureau of Liaoning Province (No. 2009A451)
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