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Effects of tumor cells on lipid contents and protein secondary structures of dendritic cells at different differentiation stages
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Abstract:
Objective:To investigate the effect of tumor cells on lipid contents and protein secondary structures in dendritic cells (DCs) of different differentiation stages by Fourier transformed infrared spectrum (FT-IR), and to provide a clue for understanding the mechanism of tumor immune escape. Methods: CD14＋monocytes were isolated by immunomagnetic beads, and the immature DCs (imDCs) and mature DCs (mDCs) were induced by classic method. The imDCs and mDCs were co-cultured with human hepatoma BEL7402 cells, erythroleukemia K562 cells or human umbilical vein endothelial cells (HUVEC) for 48 h, untreated DCs served as control. The effects of tumor cells on lipid contents and protein secondary structures of DCs at different differentiation stages were investigated by FT-IR. Results: Compared with untreated DCs, the concentration of membrane phospholipid in DCs co-cultured with BEL7402 and K562 tumor cells was significantly decreased（2.718±0.296，3.124±0.361 vs 4.855±0.324，P<0.05; 2.964±0.136，3.522±0.173 vs 4.217±0.206，P<0.05）, the total lipid was increased （3.768±0.185，3.591±0.197 vs 2.487±0.212，P<0.05; 4.288±0.156，4.155±0.167 vs 3.233±0.206，P<0.05）, the protein α-helix was decreased （1.863±0.192，1.754±0.169 vs 2.364±0.188，P<0.05; 1.124±0.133，1.016±0.107 vs 1.392±0.113，P<0.05）， and the protein-sheet and β-turn were increased （3.397±0.225，3.433±0.236 vs 2.486±0.198，P<0.05; 2.646±0.209，2.591±0.216 vs1.558±0.159，P<0.05）. Moreover, compared with imDCs, mDCs were more susceptible to tumor-derived factors （4.366±0.284，4.322±0.266 vs 3.127±0.272，P<005; 2.675±0.221，2.627±0.235 vs 1.773±0.181，P<0.05）.Conclusion: Tumor cells can elicit the changes of lipid contents and protein secondary structures of imDCs and mDCs, which might be the structure basis for functional impairment caused by tumors.
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Project Supported:
Project supported by the Key Program of Science and Technology from Chinese Ministry of Education (No. 210196), the Governor Foundation of Guizhou Province (No. 2009-79), the Natural Science Foundation of Guizhou Province (No. J-2008-2274), and the Science and Technology Program of Guiyang Guizhou Province (No. 2010-1-12)
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