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Attenuated vesicular stomatitis virus induces apoptosis in cervical cancer HeLa cells and its mechanism
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Abstract:
Objective:To investigate the apoptosis-inducing effect of an attenuated vesicular stomatitis virus in cervical cancer HeLa cells, and to explore the possible mechanism. Methods: HeLa cells were infected with VSV (MOI=1.0) and the cell proliferation was determined by MTT assay at 6, 12, 18, 24, and 30 h after infection. Morphological changes of apoptosis were observed by acridine orange (AO)/ethidium bromide (EB) staining. Annexin V/PI double-staining was performed to detect early apoptosis rate of HeLa cells and the sub-G1 apoptotic peak was examined by flow cytometry. The mitochondrial membrane potential of HeLa cells was measured by the JC-1 staining. The activities of caspase-9, -8 and -3 were measured by caspase assay kit. Results: After HeLa cells were exposed to attenuated VSV for 12 h and 24 h, the viabilities of cells were reduced to (78.4±1.9)% and (63.1±5.6)% (P<0.01); the early apoptosis rates were (16.88±248)% and (31.9±4.24)% (P<0.01); the Sub-G1 apoptotic peaks were (14.85±1.48)% and (21.05±228)% (P<0.01), respectively. Attenuated VSV significantly decreased mitochondrial membrane potential in HeLa cells with the increase of infection time (P<0.05). The activities of caspase-9 and caspase-3 of HeLa cells were significantly increased after VSV infection (all P<0.05). Conclusion: The attenuated VSV can inhibit the proliferation of HeLa cells and trigger apoptosis via caspase-9 and caspase-3-dependent pathway.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 31001070), the Science and Technology Development Foundation of Jilin Province（No. 20100146）, and the Innovative Start-up Foundation of the Eleventh Institute of Academy of Military Medical Sciences (No.YCX0902)
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