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Abstract:
Objective:To study the effect of antisense human telomerase RNA (hTR) on adhesion and invasion of hepatocellular carcinoma (HCC) cell line BEL-7402 and its mechanism. Methods: Our previous research had successfully transfected sense or antisense hTR gene into BEL-7402 cells. This study they were divided into 3 groups: the sense hTR transfected group (BEL-7402-hTR-EcoRI), the antisense hTR transfected group (BEL-7402-hTR-BamHI) and the blank control group (BEL-7402). The adhesion and invasion of BEL-7402 cells after antisense hTR transfection were observed with adhesion and invasive assay, respectively. The expressions of integrin β1 (INTβ1) and epithelial cadherin (E-cad) were detected by immunocytochemistry, and galatin zymography was used to measure the activities of matrix metalloproteinase-2 (MMP-2) and MMP-9. Results: The adhesion and invasion abilities of antisense hTR transfected BEL-7402 cells were significantly weaker than those of the blank control and sense hTR transfected groups (0.204±0.029 vs 0.505±0037, 0.465±0.029; 34.80±3.19 vs 71.47±5.15, 69.87±3.11; all P<0.05). Meanwhile, the expression of INTβ1 was also significantly decreased (0.153 3±0.015 6 vs 0.385±0.008, 0.375±0.014 4, P<0.05), and E-cad was significantly increased (0.209±0.020 vs 0.124±0.018, 0.134±0.016; P<0.05) in the antisense hTR transfected group. The activities of MMP-2 and MMP-9 were significantly inhibited in the antisense hTR transfected group (2 05422±13852 vs 3 105.56±329.60, 2 923.22±269.08; 846.33±104.66 vs 1 538.89±122.85, 1 453.33±126.35; all P<0.05). Conclusion:Antisense hTR can inhibit the adhesion and invasion of hepatocellular carcinoma BEL-7402 cells, which may be related to the increase of E-cad expression, decrease of INTβ1 expression, and decrease of MMP-2 and MMP-9 activities.
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Project Supported:
Project supported by the Medical Science Research Foundation of Shandong Province (No. 2001CA1DBA3)
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