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Synergistic inhibitory effects of bFGF monoclonal antibody and S-1 against proliferation of lung cancer Lewis cells and angiogenesis of transplanted tumors
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Abstract:
Objective: To study the synergistic inhibitory effects of basic fibroblast growth factor (bFGF) monoclonal antibody (bFGF mAb ) and gimeracil and oteracil porassium (S-1) against proliferation of Lewis cells and the growth, metastasis, angiogenesis of the transplanted tumors. Methods: CCK-8 assay was used to assess the effects of bFGF mAb and S-1 on proliferation of Lewis cells. The spontaneous Lewis cell lung metastatic model was established, and thirty-two C57BL /6 mice were randomly divided into 4 groups: normal sodium (NS) group, bFGF mAb group, S-1 group, and bFGF mAb+S-1 group. Tumor volume was measured and tumor growth curve was drawn; tumors were weighed and the inhibitory rate of tumor growth was calculated; metastatic nodules on lung surface were counted; and the vascular endothelial cells were stained with CD31 to examine the microvessel density (MVD) of transplanted tumors. Results:Both bFGF mAb and S-1 inhibited Lewis cell proliferation in a dose-dependent manner (P<0.05). The inhibitory rate in bFGF mAb+S-1 group was significantly higher than those in the single drug treatment groups (P<0.05 or P<0.01). The inhibitory rates of transplanted tumors in bFGF mAb group, S-1 group, and bFGF mAb+S-1 groups were 37.8%, 47.7%, and 65.9%, respectively, with the combination group being significantly higher than the single treatment groups (P<0.05 or P<0.01). Moreover, the metastatic nodules and MVD in the combination group were significantly lower than those of single treatment groups (2.71±0.76 vs 6.57±0.98, 4.71±0.76; 21.6±2.9 vs 33.4±4.9, 41.9±63; P<0.05 or P<0.01). Conclusion: bFGF mAb and S-1 have synergistic inhibitory effects on Lewis transplanted tumors, which is related to the inhibition of proliferation and angiogenesis.
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Project Supported:
Project supported by the National High Technology Research and Development Program (863 Program) of China (No. 2009AA022112)
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