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Effect of gemcitabine combined with TRAIL on proliferation and apoptosis of lung cancer NCI-H460 cells
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Abstract:
Objective:To study the effect of gemcitabine combined with TRAIL (TNF-related apoptosis inducing ligand) on proliferation and apoptosis of human lung cancer NCI-H460 cells. Methods: MTT assay was used to analyze the proliferation of NCI-H460 cells, and flow cytometry was used to examine the apoptosis of NCI-H460 cells. Results: Gemcitabine or TRAIL alone or in combination inhibited the proliferation of NCI-H460 cells in a time-(24, 48, 72 h) and concentration-dependent (0.001-10 μg/ml) manner. Gemcitabine combined with TRAIL had a stronger inhibitory effect than they were used alone, with the inhibitory ratios being 14.9%-77.5%, 23.4%-74.8%, and 22.3%-805% after 72 h in combination, gemcitabine, and TRAIL groups, and the IC50 being 0.0853, 0.0982, and 0.0435 μg/ml respectively. The inhibitory effect of gemcitabine combined with TRAIL on proliferation of NCI-H460 cells was related to the administration order and the ratio of gemcitabine and TRAIL; gemcitabine treatment for 24 h and then giving TRAIL had a stronger inhibitory effect than TRAIL treatment for 24 h and then giving gemcitabine; when the ratio of gemcitabine and TRAIL was 1:0.3, the combination showed the strongest inhibitory effect. The apoptosis rate of NCI-H460 cells in the combination treatment group was significantly higher than in the other two groups (49.04% vs 29.33%, 25.69%, P<0.01). Conclusion:Gemcitabine and TRAIL have synergistic effects in inhibiting proliferation and promoting apoptosis of lung cancer NCI-H460 cells.
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Project Supported:
Project supported by Key Science and Technology Foundation of “Major New Drug Research” of China（No. 2009ZX09103-689），and the Science Foundation for Cultivating Undergraduate Innovative Ability of Second Military Medical University (No. MS2009033)
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