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Relationship of CD107a expression with cytotoxic activity of human peripheral γδT cells
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Abstract:
Objective:To investigate changes of CD107a expression in γδT cells during cultivation and the relationship of CD107a expression with cytotoxicity of γδT cells. Methods: γδT cells were generated in vitro by stimulating PBMCs with IL-2 and isopentenyl pyrophosphate (IPP). Phenotype analysis of γδT cells was performed on the 7, 10 and 14 day by flow cytometry. Meanwhile, CD107a, perforin and granzyme B expressions were detected in γδT cells by flow cytometry. The cytotoxicity of γδT cells on pancreatic carcinoma SW-1990 cells was determined by CCK-8 kit. Spearman correlation analysis was performed by SPSS13.0 software. Results: γδTCR expression in γδT cells was (60.31±3.84)%, (66.45±4.25)% and (70.99±4.66)% on 7, 10 and 14 day, respectively. The expression of CD107a, perforin and granzyme B reached the peak on 7~10 d (7 d vs 0 d: \[80.66±4.42\]%, \[70.11±3.34\]%, \[94.26±4.25\]% vs \[69.02±5.04\]%, \[62.31±4.66\]%, \[53.62±3.69\]%, P<0.05), and then gradually decreased. The cytotoxicity rates of 7 day and 10 day γδT cells against SW-1990 cells were significantly higher than those of 14 day γδT cells (\[5886±5.12\]%, \[61.53±4.69\]% vs \[40.31±4.83\]%, P<0.05). CD107a expression in γδT cells was significantly correlated with perforin, granzyme B expressions and cytotoxicity on SW-1990 cells (P<0.01). Conclusion: The expression of CD107a on human peripheral γδT cells is positively correlated with its anti-tumor effect and may serve as a marker for the cytotoxic activity of γδT cells.
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Project Supported:
Project supported by Medical Science and Technology Innovation Research Foundation of Nanjing Military Area Command (No. 08MA040)
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