Mobile website
Clinical Research
Massively parallel signature sequencing in screening differentially expressed microRNAs in human renal cell carcinoma tissues and their identification
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To screen differentially expressed microRNAs (miRNAs) in tumor and the corresponding paracancerous tissues of renal cell carcinoma (RCC) by massively parallel signature sequencing (MPSS), and to identify the expression of miR-660 in RCC. Methods: The expression of miRNAs in tumor and the corresponding paracancerous tissues of 10 RCC patients was examined by MPSS, and the differentially expressed miRNAs were screened. Expression of miR-660 in tumor tissues of 5 patients and the paracancerous tissues of 40 RCC patients were further examined by RT-PCR and qPCR, respectively. Results: The MPSS results showed that 283 miRNAs were up-regulated and 187 were down-regulated in RCC tissues, and the expression level of miR-660 in RCC tissues was only 19.5% of that in the paracancerous tissues. The MPSS results were primarily confirmed by RT-PCR, and qPCR results further showed that miR-660 expression in 34 of 40 RCC tissues was significantly lower than that in the paracancerous tissues, with the average level being only 11.7% of that in the paracancerous tissues. Conclusion: A total of 283 miRNAs are up-regulated and 187 down-regulated in RCC tissues; and miR-660 is lowly expressed in RCC, which may be a new target for the diagnosis and treatment of RCC.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 900817), and the Shenzhen-HongKong Innovation Circle Project (No. ZYB200907080110A)
Clc Number:
