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Effect of heparanase antisense oligodeoxynucleotide on adhesion, invasion and apoptosis of lung cancer A549 cells
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Abstract:
Objective : To study the effects of heparanase antisense oligodeoxynucleotide (HPSE-ASODN) on adhesion, invasion and apoptosis of human lung cancer A549 cells. Methods: HPSE specific ASODN was designed, synthesized, and transfected into A549 cells by lipofectin assay. HPSE protein expression was detected by Western blotting analysis in A549 cells, and the effects of HPSE-ASODN on adhesion and invasion of A549 cells were measured by adhesion and invasion assays, respectively. Hoechst 3222 staining was used to detect the effect of HPSE-ASODN on apoptosis of A549 cells. Results: Compared with control and lipofectin, HPSE-ASODN transfection inhibited HPSE protein expression, adhesion and invasion of A549 cells (P<0.01). HPSE-ASODN transfection induced apoptosis of A549 cells, with the apoptotic rate being significantly higher than those in the control and lipofectin groups (\[44.7±18.9\]% vs \[1.2±33\]%, \[5.8±20.1\]%, P<0.01). Conclusion: HPSE-ASODN can inhibit HPSE protein expression, adhesion and invasion in lung cancer A549 cells; it can also induce the apoptosis of lung cancer A549 cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30571844), and the Natural Science Foundation of Shandong Province (No. 2009ZRB14005)
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