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Cytotoxicity effect of DC-CIK on acute myelogenous leukemic stem cells
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Abstract:
Objective : To investigate the cytotoxicity effect of homologous cytokine-induced killer cells (CIKs), which was co-cultured with dendritic cells (DCs), against leukemic stem cells (LSCs) of acute myelogenous leukemic KG-1a cells. Methods: The peripheral blood mononuclear cells were isolated from healthy donors, and the adherent cells were induced to differentiate into DCs with GM-CSF and IL-4; the suspension cells were induced to differentiate into CIK cells with IL-1, IL-2, IFN-γ and CD3 mAb. KG-1a cells were frozen-thawed and the lysate antigen was obtained; the DCs pulsed with or without lysate antigen were co-cultured with CIK (Ag-DC-CIK, DC-CIK groups), and the ratios of CD34+CD38-CD123+LSCs were measured by flow cytometry in different groups; and CIK cultured alone served as blank control. DC-CIK or Ag-DC-CIK was further co-cultured with KG-1a cells, and the apoptotic rates of KG-1a and CD34+CD38-CD123+ cells were also examined by flow cytometry in different groups. Results: DCs were successfully induced from the peripheral blood mononuclear cells. The ratios of CD3+CD56+ cells in CIK, DC-CIK and Ag-DC-CIK groups were sequentially increased to (17.36±4.44)%, (28.22±3.66)%, and (36.16±5.88)%, respectively (P<001). Compared with the control group, the ratio of CD34+CD38-CD123+ in DC-CIK and Ag-DC-CIK groups were significantly decreased (\[3.95±0.53\]%, \[3.03±0.62\]% vs \[8.78±0.62\]%, P<0.01). DC-CIK induced apoptosis of KG-1a cells, with the apoptotic rate increased from (2.34±0.74)% to (12.27±1.01)%, but it showed no evident effect on apoptosis of CD34+CD38-CD123+ cells. Conclusion: DCs co-cultured with CIK can effectively kill acute myelogenous leukemic stem cells, but have no evident proapoptosis effect.
Keywords:
dendritic cell, cytokine induced killer cell, acute myelogenous leukemic stem cell
Project Supported:
Project supported by the Science and Technology supporting Program of Gansu Province（No.0804NKCA115）
