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Cytotoxic activity of IL-15 gene modified-NK cells against primary ovarian cancer cells
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Abstract:
Objective : To explore the cytotoxic activity of IL-15 gene modified-NK cells (NK-ustc cells) against primary ovarian cancer cells in vitro and in vivo. Methods: Primary ovarian cancer cells were isolated from ascites of patients. NK-ustc cells were irradiated with different dosages of gamma ray (0, 1, 2, 4, 8, 16 Gy), and the proliferation of irradiated NK-ustc cells were detected by 3H-TdR incorporation assay. Cytotoxic activities of NK-ustc cells against K562 and primary ovarian cancer cells were measured by 51Cr release assay. The tumor-bearing mouse model was established using primary ovarian cancer cells and randomly divided into NK-ustc treatment group (intraperitoneal injection of 8 Gy irradiated NK-ustc cells) and medium control group; moreover, blank control group (8 Gy irradiated NK-ustc cells were injected into nude mice) was also included in the present study. The body weight, abdomen circumference and survival time of nude mice were monitored. Results: After 1, 2, 4, 8 and 16 Gy irradiation, the proliferation rates of NK-ustc cells were (62.1±98)%, (41.3±8.7)%, (14.6±4.1)%, (0.1±0.03)% and (0.2±0.04)%, respectively. The cytotoxic rates of 0 and 8 Gy irradiated-NK-ustc cells against K562 cells were (45.4±8.9)% and (43.1±6.4)% when the effector to target ratio was 10 ∶1, and those against ovarian cancer cells were (54.6±6.4)% and (48.3±5.8)%, respectively. Thus, irradiation had no influence on cytotoxicity of NK-ustc cells (P>0.05). The median survival time of irradiated-NK-ustc cells treated mice was 75 d, and that of control group was 39 d (P<0.05). All the mice in the blank control group survived. Conclusion: Gamma ray irradiation can effectively inhibit proliferation of NK-ustc cells, but retain their cytotoxic activities against primary ovarian cancer cells.
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Project Supported:
Project supported by the Major Science and Technology Program of Anhui Province (No. 08010302101)
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