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Effects of estrogen receptor subtype on growth of breast cancer MCF7 cells and Th balance in microenvironment
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Abstract:
Objective:To study estrogen receptor (ER) subtype on the growth of breast cancer cell line MCF7 and the secretion of Th1 and Th2 cytokines in MCF7 tumor microenvironment. Methods: ERα or ERβ expression in MCF7 cells was silenced by RNA interference and MCF7 cells with different ERα/ERβ expression status were obtained. MTT test, flow cytometry and RTPCR assay were used to detect proliferation, cell cycle and expression of apoptosis suppressor genes. Secretion of IFNγ and IL4 in cell supernatant were analyzed by ELISA assay. Results: After RNA interference, protein levels of ERα or ERβ in MCF7 cells decreased by (77.7± 3.3 )% or (68.3±21)%, respectively. Compared to control group, after knocking down ERα gene expression, MCF7 cells grew slower (P＜0.05) and were arrested at phase G0～G1, expression of apoptosis suppressor gene XIAP decreased by (43.0±2.0)%. and the level of IFNγ increased by (1.89±0.34) times. However, after knocking down the ERβ gene expression, MCF7 grew faster (P<005), and the proportion of cells entering S phase increased, the expression of apoptosis suppressor genes Bcl2, Bclxl and XIAP increased by (1.28±0.21) times, (1.61±0.32) times and (1.65±0.29) times, respectively, while the level of IFNγ decreased by (28.0±4.0)%, compared to the control group. Conclusion: The expression status of ER subtype can affect the growth of MCF7 cells and induce the Th bias in microenvironment by regulating the autocrine level of IFNγ.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81041071), the Natural Science Foundation of Tianjin (No.08JCYBJC06900), and the Science and Technology Research Program of Medical College of Chinese People’s Armed Police Forces (No.WY200802)
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