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Celecoxib induces apoptosis and autophagy of gastric cancer SGC7901 cells
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Abstract:
Objective:To observe the effect of celecoxib on apoptosis and autophagy of human gastric cancer cell line SGC7901, and to investigate the mechanism of apoptosis. Methods: SGC7901 cells were treated with different concentrations of celecoxib, proliferation of SGC7901 cells was studied by MTT assay, apoptosis was assessed by TUNEL, ultrastructure changes was observed by transmission electron microscopy, apoptotic rate was examined by flow cytometry, and expression of caspase8, caspase9 mRNA was analyzed by realtime quantitative PCR. Results: Celecoxib inhibited proliferation of SGC7901 cells in a time and dosedependent manner, with inhibitory rate of (85.6±451)% at 125 μmol/L celecoxib for 72 h. Celecoxib induced apoptosis of SGC7901 cells, typical apoptotic body and autophagosome were observed under TEM, and apoptotic rate increased from (2.2±1.32)% to (35.7±5.73)% (P<0.05) as detected by FCM. Expression of caspase8 and caspase9 mRNA increased sharply in SGC7901 cells treated with celecoxib in a time and dosedependent manner. Conclusion: Celecoxib can induce apoptosis of gastric cancer SGC7901 cells by activating caspase8 in the deathreceptor pathway and caspase9 in the mitochondrial pathway, and induce autophagic cell death.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30872478), and the Technology Research and Development Special Programs of Gansu Province (No. 0912TCYA027)
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