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Membrane-bound IL-15 combined with RAE-1ε enhances cytotoxicity of mouse NK cells
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Abstract:
Objective : To study the effects of membrane-bound IL-15 in combination with retinoic acid early transcript 1ε (RAE1ε) on cytotoxicity of NK cells. Methods: Three derivatives of mouse pro-B lymphocyte cell line BaF3, expressing membrane-bound IL-15 (termed BaF3/mb15), or RAE1ε (termed BaF3/RAE), or both membrane-bound IL-15 and RAE1ε (termed BaF3/mb15/RAE) were respectively constructed in the previous study. Mouse NK cells were isolated and stimulated with irradiated BaF3 derivatives. Phenotype analysis of NK cells was performed by flow cytometry. Meanwhile, perforin and granzyme B expressions were detected in NK cells by intracellular staining; the cytotoxicity of NK cells against YAC1 lymphoma target cells was evaluated by flow cytometry. Results: NK cells stimulated with BaF3-mb15-RAE cells expressed higher levels of CD25, CD44, FasL and CD107a compared to NK cells stimulated with BaF3/mb15 or BaF3/RAE cells. However, BaF3-mb15-RAE cells showed no effects on perforin and granzyme B production of NK cells. When the effector to target ratio was 20∶1, the cytotoxicity rates of BaF3/mb15-stimulated NK cells, BaF3/RAE-stimulated NK cells and BaF3/mb15/RAE-stimulated NK cells against YAC1 cells were (39.7±2.9)%, (45.3±23)% and (59.0±6.9)%, respectively, and significantly increased compared with that of BaF3-stimulated NK cells （\[28.3±1.5\]%, P<0.01). Furthermore, BaF3/mb15/RAE-stimulated NK cells exhibited higher cytotoxicity on YAC1 cells than BaF3/mb15-stimulated or BaF3/RAE-stimulated NK cells (P<0.05). Conclusion: IL-15 combined with RAE1ε promotes the activation and cytotoxicity of NK cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81001308, No.30800182, No.31100619), the Natural Science Foundation of Jiangsu Province (No.BK2010315), and the “Chen-guang Program” of Shanghai Municipal Education Commission (No.11CG48)
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