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MicroRNA-10a promotes invasion of glioma cells by regulating MMP expression
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Abstract:
Objective :To investigate the effect of microRNA-10a (miR-10a) on invasion of glioma cell line U87MG. Methods: miR-10a antisense oligodeoxynucleotides (miR-10a-anti-ODN) enveloped with liposome were transfected into glioma cell line U87MG, and nonsense miRNA and blank control were used as control groups. Flow cytometry and fluorescence microscope were employed to determine the transfection efficiency of miR-10a-anti-ODN in U87MG cells; flow cytometry was employed to detect the apoptosis and cell cycle of U87MG cells after miR-10a-anti-ODN transfection; MTT assay were used to detect the proliferation of U87MG cells; Transwell assay were applied to ascertain effect of miR-10a-anti-ODN on migration and invasion of U87MG cells; and RT-PCR and Western blotting were used to examine expressions of mRNA and protein of MMP-2, MMP-9 and MMP-14 . Results: miR-10a-anti-ODN transfection had no obvious effect on proliferation, cell cycle and apoptosis of U87MG cells; however, invasion and migration of U87MG cells was significantly decreased (invasion: \[87±7.1\] vs \[155±3.7\], \[149±6.6\], P<0.05; migration: \[78.0±5.2\] vs \[150. 3±3.7\] \[147.3±6.6\]), and mRNA and protein expressions of MMP-2, MMP-9 and MMP-14 were also significantly decreased. Conclusion: miR-10a can promote invasion of glioma cell line U87MG through upregulating MMP-2, MMP-9 and MMP-14 expressions, which might be a target in glioma therapy.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30672165, No. 30872657), the “333” Key Talent Foundation of Jiangsu Province (No. 0508RS08), and the Medical Major Talent Foundation of Jiangsu Province (No. RC2007061)
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