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Effect of radiation-induced oncolytic adenovirus combined with chemotherapy on cervical cancer HeLa S3 cells
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Abstract:
Objective : To construct a new radiation-induced, EGR-1 promotor-regulated, human TRAIL gene containing oncolytic adenovirus Ad-EGR-TRAIL, and to investigate the cytotoxicity effect of Ad-EGR-TRAIL combined with chemotherapy on cervical cancer HeLa S3 cells. Methods: Recombinant adenovirus Ad-EGR-TRAIL was constructed. HeLa S3 cells were infected with Ad-GFP, and infection efficiency was observed. The cytotoxicity effect of Ad-EGRTRAIL, radiotherapy (RAD), and Ad-EGR-TRAIL+RAD on HeLa S3 cells, as well as on normal human cervical cells, was examined by CCK-8 method. Results: Recombinant adenovirus Ad-EGR-TRAIL was successfully constructed. Ad-EGR-TRAIL showed the highest infection efficiency at MOI=100 in HeLa S3 cells. The inhibitory rates of HeLa S3 cells were (8.07±3.02)% and (23.02±4.03)% when Ad-EGR-TRAIL or RAD was used alone; however, the inhibitory rate reached (79.77±9.15)% when Ad-EGR-TRAIL and RAD were used in combination; and normal cervical cells did not significantly respond to the combination Ad-EGR-TRAIL and RAD therapy. Conclusion: Ad-EGR-TRAIL combined with chemotherapy can significantly kill cervical cancer HeLa S3 cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.10979034, No.31071228), and the Natural Science Foundation of Shanghai (No. 09ZR1416400)
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