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Chemotaxis of bone marrow-derived mesenchymal stem cells to gastric cancer cells in vivo and in vitro
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Abstract:
Objective :To investigate the tropism of human bone-derived mesenchymal stem cells (hBMSCs) to gastric cancer cells in vitro and in vivo and to provide evidence for MSC as the vehicle for gene therapy of gastric cancer. Methods: hBMSCs were isolated and cultured using bone marrow plating method, and identified by flow cytometry. Migration abilities of hBMSCs to gastric cancer MKN45 cells and human fibroblast (hFB) cells were examined by Transwell assay. the tumor nude mice model transplanted by MKN45 cells was established. And Lenti-EGFP infected-hBMSC or-hFB (MOI=50) cells were subcutaneously injected into tumor-bearing mice, then GFP expression in transplanted tumors and different organs were observed under a fluorescence microscope. Results: The positive expression rates of CD44 and CD105 in the third passage hBMSC were (96.7±1.84)% and (98.1±0.95)% respectively, while CD34 and CD45 were negatively expressed. Chemotaxis of hBMSC to MKN45 cells was significantly higher than that to gastric epithelial GES-1 cells (239.5±54.3 vs 43.57±4.6, 37.3±4.7; P<0.01), and chemotaxis of hBMSC to MKN45 cells was significantly higher than that of hFB cells (P>0.05). hBMSC migrating to MKN45 were significantly higher than hFB (239.5±54.3 vs 27.7±16.7, P<0.01). Compared with hFB cells, hBMSC showed obvious chemotaxis to gastric transplanted tumor tissues; in hBMSC group, GFP was highly expressed in transplanted tumor tissues, as well as in liver (20%) and lung tissues (20%), but the later were significantly lower than that in transplanted tumor tissues (P<005). Conclusion: The chemotaxis of hBMSC to gastric cancer is obvious and specific, and hBMSC may be a good vehicle for gene therapy of gastric cancer.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81060198), the Foundation from Education Bureau of Jiangxi Province (No. GJJ09429), and the Foundation from Science and Technology Bureau of Jiangxi Province (No. 2010BSA12800)
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