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Efficacy of iodinate oil magnetic paclitaxel albumin nanoparticle on rat liver cancers
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Abstract:
Objective : To prepare anti-cancer targeting drug paclitaxel albumin magnetic nanoparticles (MAG-TAX-NP) and iodinate oil MAG-TAX-NP, and study the efficacy of iodinate oil MAG-TAX-NP on rat liver cancer. Methods: MAG-TAX-NP was prepared by emulsion-ultrasonic-solidification heat method using albumin and nano-Fe3O4 particles as carriers, and then iodinate oil MAG-TAX-NP was constructed. The content of paclitaxel was detected by UV spectrophotometry, and the loading rate and the entrapment efficiency of MAG-TAX-NP were calculated. The morphology of MAG-TAX-NP was observed under electron microscope. Rat liver tumor model was established. In addition to the blank group, 0.2 ml of iodinate oil, iodinate oil paclitaxel, iodinate oil magnetic nanoparticle, or iodinate oil MAG-TAX-NP was separately injected into the hepatic artery of tumor-bearing rats. Tumor-bearing rats were sacrificed 14 d after injection, the liver tumors were weighed and the tumor inhibitory rates in each group were calculated. The inhibitory rate of Iodinate oil MAG-TAX-NP on liver tumors was observed under an electron microscope. Results: The prepared MAG-TAX-NP was smooth; its diameter was about 70 nm; its average loading rate was 5.62%; and its average entrapment efficiency was 8072%. The tumor inhibitory rate of iodinate oil MAG-TAX-NP treatment group was significantly increased (P<0.01), with tumor inhibitory rates of iodinate oil, iodinate oil paclitaxel, iodinate oil magnetic nanoparticle, and iodinate oil MAG-TAX-NP treatment groups being (43.2±2.24)%, (51±3.33)%, (57.4±3.66)%, and (87.4±4.11)%, respectively. Furthermore, liver tumor cells showed lots of apoptosis and necrosis after iodinate oil MAG-TAX-NP treatment. Conclusion: Iodinate oil MAG-TAX-NP can inhibit growth of rat liver tumors, which might become a novel paclitaxel formulation in treatment of liver cancers.
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Project Supported:
Project supported by the Key Program of Science and Technology of Heilongjiang Province（No. GB05C 402-07）, and the Program of Science and Technology of Education Bureau of Heilongjiang Province（No. 11521183）
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