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Comparison of loss of heterozygosity at chromosomal microsatellites in invasive ductal carcinoma and ductal carcinoma in situ of breast
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Abstract:
Objective : To explore the loss of heterozygosity (LOH) of microsatellite sites at chromosome 3p in ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) lesions. Methods: Forty-three paraffin blocks were obtained from patients with invasive ductal carcinoma who received surgery (Sep. 2005 to Feb. 2009, Changhai Hospital). The IDC, DCIS and normal tissues were microdissected from paraffin sections. The LOH of 4 microsatellite sites was examined with the application of capillary electrophoresis sequencing techniques. Results: In 40 breast cancer cases, the frequencies of LOH at D3S1038, D3S1295, D3S1581 and D3S3118 were 25% (8/40), 37.5% (15/40), 17.5% (7/40) and 5% (2/40), respectively. The frequency of LOH in IDC group was higher than that in DCIS group with no significant difference (47.5% vs 37.5%). Conclusion: LOH at chromosome 3P site is an early event in the development of breast ductal carcinoma. The frequency of LOH in DCIS is close to that in IDC. In the development of breast carcinoma, new MS sites have LOH.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30870975)
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