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Monographic Report
Effect of stimulation with combined IL-12 and IL-18 on exosomes secreted by dendritic cells
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Abstract:
Objective : To observe the effect of stimulation with combined IL-12 and IL-18 on exosomes secreted by dendritic cells （DCs）, and so as to facilitate exploring high-efficiency DC derived exosome(Dex) cancer vaccines. Methods: DCs induced from human peripheral blood mononuclear cells (PBMCs) were stimulated with IL-12+IL-18，IL-12 or IL-18 for the combined group，IL-12 group and IL-18 group respectively; and no stimulant for the control group. Dex was extracted from DCs. The expressions of HLA-DR and CD83 in Dex were detected by Western blotting. CD54, CD80 and CD86 expressions were determined by flow cytometry. The T cell proliferation stimulated by Dex was detected with MTT method. IFN-γ in the T cell supernatant was detected by ELISA. Results: HLA-DR and CD83 proteins were expressed in Dex of all the four groups. The molecule expressions for combined group were CD54( 32367± 44.06 vs 246.17±31.91, 236.33±33.87, 167.67±28.73, P<0.05), CD80（406.37±39.18 vs 331.67±3615, 335.67±41.38, 260.00±35.58, P<0.05), and CD86（390.50±38.06 vs 314.33±36.64, 319.00± 33.10, 246.83± 30.55, P<0.05), which were higher than those in the IL-12 group, IL-18 group and control group. The T cell proliferation in the combined group （1.98±0.31 vs 1.55±0.23, 1.57±0.21, 1.10±0.18, P<005) was higher than that in the IL-12 group, IL-18 group and control group. IFN-γ secreted by T cells in the combined group （436.67±61.8 vs 295.04±40.25, 358.18±55.77, 225.00±36.44, P<0.05) was higher than that in the IL-12 group, IL-18 group and control group. Conclusion: Stimulation of DCs with combined IL-12 and IL-18 can enhance Dex express CD54, CD80 and CD86, promote Dex-induced T cell proliferation and increase IFN-γ secretion by T cells.
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Project supported by the Natural Science Foundation of Shandong Province (No.Y2007C111）
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