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Monographic Report
Cytotoxic effect of CTLs elicited by dendritic cells infected with adenovirus containing EPHA2 gene on glioma cells
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Abstract:
Objective : To explore the cytotoxic effect of cyctotoxic T lymphocytes (CTLs) induced by dendritic cells (DCs) modified by EPHA2 gene on U251 glioma cells, and to provide new ways for glioma immune therapy. Methods: DCs originated from the HLA-A2 +PBMCs were infected with recombinant adenovirus containing EPHA2 full length cDNA, and the DC vaccine modified by EPHA2 gene was prepared. The expression of EPHA on DCs was detected by Western blotting and FACS. HLA-A2 +PBMCs were stimulated by the DC vaccine in vitro. The specificity CTL activity induced by rAd- EPHA2 -DCs and the cytotoxicity on HLA-A2 +U251 glima cells were detected by enzyme-linked immunospot assay (ELISPOT) and standard 51 Cr release experiment. Results: The DC vaccine modified by EPHA2 gene was successfully prepared, and EPHA2 protein was effectively expressed. Compared to DCs infected with rAd-LacZ and PBS groups, DCs infected with rAd-EPHA2 stimulated CTL activity effectively (\[187±21\] vs \[12±4\], \[18±5\]; P<0.01) and the CTLs induced by rAd-EPHA2-DCs produced cytotoxicity effect on U251 cells obviously (\[45.7±6.8\]% vs \[7.1±45\]%, P<0.01), and did not cause the cytotoxicity of its own lymphocytes. Conclusion: The DC vaccine modified by EPHA2 gene can stimulate the specificity CTL response effectively, and can cause cytotoxicity on glioma U251 cells obviously.
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Project Supported:
Project supported by the Natural Science Foundation of Fujian Province (No.2006J0373)
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