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Trichostatin A induces apoptosis of endometrial cancer Ishikawa cells by up- regulating expression of Krupell-like factor 4
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Abstract:
Objective : To observe the effect of Trichostatin A (TSA) on the apoptosis of endometrial cancer Ishikawa cells and to study its relationship with Krupell-like-factor 4 (KLF4) in this course. Methods: Ishikawa cells were cultured with different concentrations of TSA 0, 50, 100, 200, 300, 500 ng/ml for 24 h or 100 ng/ml TSA for 0, 4, 8, 12, 24 and 48 h. FACS and qRT-PCR were used to detect apoptosis and KLF4 mRNA level, respectively. Results: The apoptosis rate was increased compared to the control in the Ishikawa cells treated with 100 ng/ml TSA for 24 h （\[30.6±45\]%vs \[7.53±0.93\]%, P<0.05). The mRNA levels of KLF4 were up-regulated after Ishikawa cells were stimulated with different concentrations of TSA for 24 h or with 100 ng/ml TSA for 4, 8, 12, 24, 48 h (P<0.05). Those effects were in a dose-dependent or time-dependent manner. The apoptosis rate was increased compared to the control in the Ishikawa cells over-expressed KLF4 （\[27.3±27\]% vs \[4.53±1.75\]%, P<0.05). Conclusion: TSA induces apoptosis of Ishikawa cells by up-regulating the expression of KLF4.
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Project supported by the National Major Basic Research Program (973 Program) of China (No.2010CB529905)
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